Search results for "2 -O-methylation"

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Next‐Generation Sequencing‐Based RiboMethSeq Protocol for Analysis of tRNA 2′‐O‐Methylation

2017

Analysis of RNA modifications by traditional physico‐chemical approaches is labor  intensive,  requires  substantial  amounts  of  input  material  and  only  allows  site‐by‐site  measurements.  The  recent  development  of  qualitative  and  quantitative  approaches  based  on   next‐generation sequencing (NGS) opens new perspectives for the analysis of various cellular RNA  species.  The  Illumina  sequencing‐based  RiboMethSeq  protocol  was  initially  developed  and  successfully applied for mapping of ribosomal RNA (rRNA) 2′‐O‐methylations. This method also  gives excellent results in the quantitative analysis of rRNA modifications in different species and  under varying growth condi…

0301 basic medicine2 -O-methylationSaccharomyces cerevisiaelcsh:QR1-502Biochemistrylcsh:MicrobiologyDNA sequencingdeleted strain03 medical and health sciences[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] deleted strainTrmH 2′‐O‐methylationMolecular BiologytRNAIllumina dye sequencingRiboMethSeq TRM3Genetics RiboMethSeq030102 biochemistry & molecular biologybiologytRNA; 2′‐O‐methylation; RiboMethSeq; high‐throughput sequencing; deleted strain;  TrmH; TRM32'-O-methylationRNAhigh-throughput sequencing[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyMethylation  TrmHRibosomal RNAbiology.organism_classification030104 developmental biology high‐throughput sequencingTRM3Transfer RNA
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